O-058 Metabolism, histone modification and early embryo development

Abstract Cellular metabolism provides fundamental components for chromatin dynamics and epigenetics. However, metabolic features, their impact on epigenetic remodeling during mammalian pre-implantation development, remain poorly understood. In this study, we established the landscape of mouse embryos from zygote to blastocyst, quantified absolute carbohydrate metabolites. We found that amino acid/carbohydrate/lipid increased whereas nucleotide at 4- 8-cell stages. further demonstrated oxidized nicotinamide adenine dinucleotide (NAD+) is indispensable development. Mechanistically, NAD+ avoids excessive minor zygotic gene activation (ZGA) by cooperating with deacylase SIRT1 remove H3K27ac. human, supplement can promote removal H3K27ac benefit preimplantation Our findings demonstrate precise timely regulation ZGA controlled dynamics, enhance our understanding early embryos.